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	Provedor de dados Genet. Mol. Biol. (2) Autor Oliveira,Marcos A. (2) Pereira,Gonçalo A.G. (2) Carraro,Dirce M. (1) Cunha,Anderson F. (1) Genu,Victor (1) Reis,Sérgio F. dos (1) Salmazo,Anita P.T. (1) Souza,Franco L. (1) Mais... Palavra-chave Conservation (1) Dispersal (1) Gene flow (1) Glucose repression (1) Hansenula polymorpha (1) Hydromedusa maximiliani (1) MOX (1) SNF1 (1) TUP1 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Estimating dispersal and gene flow in the neotropical freshwater turtle Hydromedusa maximiliani (Chelidae) by combining ecological and genetic methods Genet. Mol. Biol. Souza,Franco L.; Cunha,Anderson F.; Oliveira,Marcos A.; Pereira,Gonçalo A.G.; Reis,Sérgio F. dos. Hydromedusa maximiliani is a vulnerable neotropical freshwater turtle endemic to mountainous regions of the Atlantic rainforest in southeastern Brazil. Random amplified polymorphic DNA (RAPD) was used to estimate the gene flow and dispersal for individuals inhabiting rivers and streams within a drainage. Nine primers generated 27 scoreable bands, of which 9 (33%) were polymorphic and produced 12 RAPD phenotypes. The gene flow estimates (Nm) among turtles inhabiting different rivers and streams were variable, ranging from 0.09 to 3.00 (mean: 0.60). For some loci, the rates of gene flow could offset population differentiation (Nm > 1), whereas for others random genetic drift could result in population divergence (Nm < 1). Since the genetic variation of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydromedusa maximiliani; Dispersal; Gene flow; Conservation. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200007 The transcription factor Snf1p is involved in a Tup1p-independent manner in the glucose regulation of the major methanol metabolism genes of Hansenula polymorpha Genet. Mol. Biol. Oliveira,Marcos A.; Genu,Victor; Salmazo,Anita P.T.; Carraro,Dirce M.; Pereira,Gonçalo A.G.. Hansenula polymorpha is a methylotrophic yeast widely employed in biotechnology as a ''protein factory''. Most promoters used for heterologous protein expression, like MOX (methanol oxidase) and DAS (di-hydroxy acetone synthase), are involved in the peroxisomal methanol metabolism (C1 metabolism) and are under strong glucose repression. Interestingly, the MOX promoter is subjected to glucose regulation also in Saccharomyces cerevisiae, a non-methylotrophic yeast in which this phenomenon is well studied. In this species, the transcription factor Tup1p plays an essential role in glucose repression of several genes. This effect is counteracted by the activator Snf1p when glucose is exhausted from medium. Therefore, to test whether this regulatory circuit has... Tipo: Info:eu-repo/semantics/article Palavras-chave: TUP1; SNF1; Hansenula polymorpha; Glucose repression; MOX. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400017 Registros recuperados: 2 Primeira ... 1 ... Última

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